Destroy the 5 hidden target diamonds in Act 4: The Level Factory

Fire 40 paintballs in under 5 seconds

Ace Act 5: The Boss

Total survivors rescued has surpassed 10,000

Stage 1-1 cleared

Fire 5,000 paintballs

Play five multiplayer games

The player has become a Big Daddy

The player has either Harvested or Rescued every possible Little Sister

The player has successfully invented all possible ammo types

The player has fully upgraded three weapons

The player has purchased every slot in one of the Plasmid or Tonic tracks